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UniProt functional annotation for Q9LFT6 | ||
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| UniProt code: Q9LFT6. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Involved in cyanogenesis, the release of HCN from injured tissues (By similarity). Displays R-selective hydroxynitrile lyase activity. Also accepts nitromethane (MeNO2) as a donor in a reaction with aromatic aldehydes to yield (R)-beta-nitro alcohols. {ECO:0000250, ECO:0000269|PubMed:17907254, ECO:0000269|PubMed:18467465, ECO:0000269|PubMed:19433222, ECO:0000269|PubMed:21439333}. |
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| Catalytic activity: | |
Reaction=(R)-mandelonitrile = benzaldehyde + hydrogen cyanide; Xref=Rhea:RHEA:18313, ChEBI:CHEBI:17169, ChEBI:CHEBI:18407, ChEBI:CHEBI:18450; EC=4.1.2.10; Evidence={ECO:0000269|PubMed:17907254}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.4 mM for mandelonitrile {ECO:0000269|PubMed:19433222}; |
| Subunit: | |
Homodimer. {ECO:0000250}. |
| Similarity: | |
Belongs to the AB hydrolase superfamily. Hydroxynitrile lyase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.