PDBsum entry 6bf8

Biosynthetic protein

PDB id: 6bf8

Contents

Protein chains

949 a.a.

PDB id:

6bf8

Name:

Biosynthetic protein

Title:

Cryo-em structure of human insulin degrading enzyme in complex with insulin

Structure:

Insulin-degrading enzyme. Chain: a, b. Fragment: residues 46-1011. Synonym: abeta-degrading protease,insulin protease,insulinase, insulysin. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ide. Expressed in: escherichia coli. Expression_system_taxid: 562

Authors:

W.G.Liang,Z.Zhang,L.J.Bailey,A.A.Kossiakoff,Y.Z.Tan,H.Wei, B.Carragher,S.C.Potter,W.J.Tang

Key ref:

z.zhang et al. (2018). Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme. Elife, 7, . PubMed id: 29596046

Date:

26-Oct-17 Release date: 04-Apr-18

Protein chains

P14735  (IDE_HUMAN) -  Insulin-degrading enzyme from Homo sapiens

Seq: 1019 a.a.

Struc: 949 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.24.56 - insulysin.
• Reaction: Degradation of insulin, glucagon and other polypeptides. No action on proteins.
• Cofactor: Zn(2+)

Elife 7: (2018)

PubMed id: 29596046

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme.

z.zhang, g.w.liang, j.l.bailey, y.z.tan, h.wei, a.l.mccorda.woods, m.farcasanu, a.wang, d.lee, w.shang, c.s.meredithr.deprez-poulain, b.deprez, d.liu, s.koide, a.a.lissiakofb.carraghter, s.c.potter, w.j.tang.

ABSTRACT

No abstract given.