EC 188.8.131.52 - Insulysin
IntEnz Enzyme Nomenclature
insulin-degrading neutral proteinase
- Degradation of insulin, glucagon and other polypeptides. No action on proteins.
A 110 kDa cytosolic enzyme, known from mammals and the fruit fly, Drosophila melanogaster. Inhibited by bacitracin, chelating agents EDTA and 1,10-phenanthroline, and by thiol-blocking reagents such as N-ethylmaleimide, but not by phosphoramidon. In peptidase family M16 (pitrilysin family). Formerly EC 184.108.40.206, EC 220.127.116.11 and EC 18.104.22.168.
Links to other databases
Insulin degradation: mechanisms, products, and significance.Endocrine Rev. 9: 319-345 (1988). [PMID: 3061785]
Insulin-degrading enzyme: stable expression of the human complementary DNA, characterization of its protein product, and chromosomal mapping of the human and mouse genes.Mol. Endocrinol. 4: 1125-1135 (1990). [PMID: 2293021]
Human red blood cell insulin-degrading enzyme and rat skeletal muscle insulin protease share antigenic sites and generate identical products from insulin.J. Biol. Chem. 265: 2984-2987 (1990). [PMID: 1689296]
Cloning and expression of the cDNA for a Drosophila insulin-degrading enzyme.Mol.Endocrinol. 4: 1580-1591 (1990). [PMID: 2126597]
Comparison of the enzymatic and biochemical properties of human insulin-degrading enzyme and Escherichia coli protease III.J. Biol. Chem. 267: 2414-2420 (1992). [PMID: 1733942]
[EC 22.214.171.124 created 1972 as EC 126.96.36.199, transferred 1976 EC 188.8.131.52, transferred 1978 to EC 184.108.40.206, transferred 1993 to to EC 220.127.116.11 (EC 18.104.22.168 created 1992, incorporated 2000)]