PDBsum entry 5vev

Ligase

PDB id: 5vev

Contents

Protein chains

406 a.a.

Ligands

ACT ×3

EDO ×13

SO4 ×3

Metals

_NA ×2

Waters ×552

PDB id:

5vev

Name:

Ligase

Title:

Crystal structure of phosphoribosylamine-glycine ligase from neisseria gonorrhoeae

Structure:

Phosphoribosylamine--glycine ligase. Chain: a, b. Synonym: gars, glycinamide ribonucleotide synthetase, phosphoribosylglycinamide synthetase. Engineered: yes. Mutation: yes

Source:

Neisseria gonorrhoeae (strain nccp11945). Organism_taxid: 521006. Strain: nccp11945. Gene: purd, ngk_2312. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.90Å R-factor: 0.167 R-free: 0.201

Authors:

Seattle Structural Genomics Center For Infectious Disease (Ssgcid)

Key ref:

D.M.Dranow et al. Crystal structure of phosphoribosylamine-Glycine liga neisseria gonorrhoeae. To be published, .

Date:

05-Apr-17 Release date: 12-Apr-17

Protein chains

B4RPN9  (B4RPN9_NEIG2) -  Phosphoribosylamine--glycine ligase from Neisseria gonorrhoeae (strain NCCP11945)

Seq: 423 a.a.

Struc: 406 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.6.3.4.13 - phosphoribosylamine--glycine ligase.
• Pathway: Purine Biosynthesis (early stages)
• Reaction: 5-phospho-beta-D-ribosylamine + glycine + ATP = N₁-(5-phospho-beta-D- ribosyl)glycinamide + ADP + phosphate + H⁺

5-phospho-beta-D-ribosylamine + glycine + ATP (Bound ligand (Het Group name = ACT) matches with 80.00% similarity) = N(1)-(5-phospho-beta-D- ribosyl)glycinamide + ADP + phosphate + H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site