PDBsum entry 5lhc

Hydrolase

PDB id: 5lhc

Contents

Protein chains

579 a.a.

Ligands

GOL ×4

PO4 ×4

Waters ×173

PDB id:

5lhc

Name:

Hydrolase

Title:

The structure of d456a mutant of nt.Bspd6i nicking endonuclease at 0.24 nm resolution .

Structure:

Nicking endonuclease n.Bspd6i. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Bacillus sp.. Organism_taxid: 1409. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.40Å R-factor: 0.199 R-free: 0.265

Authors:

G.S.Kachalova,A.K.Yunusova,A.N.Popov,R.I.Artyukh,T.A.Perevyazova, H.D.Bartunik,L.A.Zheleznaya

Key ref:

G.S.Kachalova et al. Structural implication of activity loss by d456a muta the nicking endonuclease nt.Bspd6i.. To be published, .

Date:

10-Jul-16 Release date: 16-Aug-17

Supersedes:

4wl5

Protein chains

Q8GCA3  (Q8GCA3_BACSP) -  Nicking endonuclease N.BspD6I from Bacillus sp

Seq: 604 a.a.

Struc: 579 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)