PDBsum entry 4wl5

Hydrolase

PDB id: 4wl5

Contents

Protein chain

594 a.a.

Waters ×38

Obsolete entry

PDB id:

4wl5

Name:

Hydrolase

Title:

Structure of d456a mutant of the nicking endonuclease nt.Bspd6i.

Structure:

Heterodimeric restriction endonuclease r.Bspd6i large subunit. Chain: a. Engineered: yes. Mutation: yes

Source:

Bacillus sp. D6. Organism_taxid: 127889. Gene: bspd6r1. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

3.15Å R-factor: 0.221 R-free: 0.287

Authors:

G.S.Kachalova,A.N.Popov,A.K.Yunusova,R.I.Artyukh,T.A.Perevyazova, L.A.Zheleznaya,H.D.Bartunik

Key ref:

G.S.Kachalova et al. Structure of d456a mutant of the nicking endonuclease nt.Bspd6i.. To be published, .

Date:

06-Oct-14 Release date: 23-Dec-15

Protein chain

A3FEV7  (A3FEV7_9BACI) -  Heterodimeric restriction endonuclease R.BspD6I large subunit from Bacillus sp. D6

Seq: 604 a.a.

Struc: 594 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)