PDBsum entry 5eoc

Immune system

PDB id: 5eoc

Contents

Protein chains

212 a.a.

216 a.a.

13 a.a.

Waters ×476

PDB id:

5eoc

Name:

Immune system

Title:

Crystal structure of fab c2 in complex with a cyclic variant of hepatitis c virus e2 epitope i

Structure:

Fab fragment (heavy chain). Chain: h. Fab fragment (light chain). Chain: l, m. Fab fragment (heavy chain). Chain: j. Ala-cys-gln-leu-ile-asn-thr-asn-gly-ser-trp-his-ile-cys. Chain: p, q. Engineered: yes

Source:

Mus musculus. Organism_taxid: 10090. Synthetic: yes. Hepatitis c virus. Organism_taxid: 11103

Resolution:

1.98Å R-factor: 0.234 R-free: 0.266

Authors:

R.Berisio,A.Ruggiero,A.Sandomenico,A.H.Patel,M.Ruvo,L.Vitagliano

Key ref:

A.Sandomenico et al. (2016). Generation and Characterization of Monoclonal Antibodies against a Cyclic Variant of Hepatitis C Virus E2 Epitope 412-422. J Virol, 90, 3745-3759. PubMed id: 26819303 DOI: 10.1128/JVI.02397-15

Date:

10-Nov-15 Release date: 10-Feb-16

Protein chains

No UniProt id for this chain

Struc: 212 a.a.

Protein chains

No UniProt id for this chain

Struc: 216 a.a.

Protein chains

P27958  (POLG_HCVH) -  Genome polyprotein from Hepatitis C virus genotype 1a (isolate H77)

Seq: 3011 a.a.

Struc: 13 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chains P, Q: E.C.2.7.7.48 - RNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate
• Enzyme class 2: Chains P, Q: E.C.3.4.21.98 - hepacivirin.
• Reaction: Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
• Enzyme class 3: Chains P, Q: E.C.3.4.22.- - ?????
• Enzyme class 4: Chains P, Q: E.C.3.6.1.15 - nucleoside-triphosphate phosphatase.
• Reaction: a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate + phosphate + H⁺
• Enzyme class 5: Chains P, Q: E.C.3.6.4.13 - Rna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1128/JVI.02397-15

J Virol 90:3745-3759 (2016)

PubMed id: 26819303

Generation and Characterization of Monoclonal Antibodies against a Cyclic Variant of Hepatitis C Virus E2 Epitope 412-422.

A.Sandomenico, A.Leonardi, R.Berisio, L.Sanguigno, G.Focà, A.Focà, A.Ruggiero, N.Doti, L.Muscariello, D.Barone, C.Farina, A.Owsianka, L.Vitagliano, A.H.Patel, M.Ruvo.

ABSTRACT

No abstract given.