PDBsum entry 5axi

Ligase/ligase inhibitor

PDB id: 5axi

Contents

Protein chains

305 a.a.

Ligands

ASP-GLY-PTR-MET-PRO

Metals

_CL ×4

_CA ×3

Waters ×167

PDB id:

5axi

Name:

Ligase/ligase inhibitor

Title:

Crystal structure of cbl-b tkb domain in complex with cblin

Structure:

E3 ubiquitin-protein ligase cbl-b. Chain: a, b, c. Fragment: unp residues 38-343. Synonym: casitas b-lineage lymphoma proto-oncogene b,sh3-binding protein cbl-b,signal transduction protein cbl-b. Engineered: yes. Cblin. Chain: e. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: cblb. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: irs-1

Resolution:

2.50Å R-factor: 0.204 R-free: 0.264

Authors:

A.Ohno,N.Maita,A.Ochi,R.Nakao,T.Nikawa

Key ref:

A.Ohno et al. (2016). Structural analysis of the TKB domain of ubiquitin ligase Cbl-b complexed with its small inhibitory peptide, Cblin. Arch Biochem Biophys, 594, 1-7. PubMed id: 26874193 DOI: 10.1016/j.abb.2016.02.014

Date:

29-Jul-15 Release date: 02-Mar-16

Protein chains

Q3TTA7  (CBLB_MOUSE) -  E3 ubiquitin-protein ligase CBL-B from Mus musculus

Seq: 982 a.a.

Struc: 305 a.a.

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine

DOI no: 10.1016/j.abb.2016.02.014

Arch Biochem Biophys 594:1-7 (2016)

PubMed id: 26874193

Structural analysis of the TKB domain of ubiquitin ligase Cbl-b complexed with its small inhibitory peptide, Cblin.

A.Ohno, A.Ochi, N.Maita, T.Ueji, A.Bando, R.Nakao, K.Hirasaka, T.Abe, S.Teshima-Kondo, H.Nemoto, Y.Okumura, A.Higashibata, S.Yano, H.Tochio, T.Nikawa.

ABSTRACT

No abstract given.