PDBsum entry 4zkf

Hydrolase

PDB id: 4zkf

Contents

Protein chain

462 a.a.

Metals

_MG ×2

Waters ×463

PDB id:

4zkf

Name:

Hydrolase

Title:

Crystal structure of human phosphodiesterase 12

Structure:

2',5'-phosphodiesterase 12. Chain: a. Fragment: unp residues 154-609. Synonym: 2-pde,mitochondrial deadenylase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: pde12. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.82Å R-factor: 0.167 R-free: 0.190

Authors:

S.Y.Kim,T.Kohno,T.Mori,K.Kitano,T.Hakoshima

Date:

30-Apr-15 Release date: 17-Jun-15

Protein chain

Q6L8Q7  (PDE12_HUMAN) -  2',5'-phosphodiesterase 12 from Homo sapiens

Seq: 609 a.a.

Struc: 462 a.a.*

* PDB and UniProt seqs differ at 6 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.3.1.13.4 - poly(A)-specific ribonuclease.
• Reaction: Exonucleolytic cleavage of poly(A) to 5'-AMP.
• Enzyme class 3: E.C.3.1.4.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.