PDBsum entry 4pf7

Hydrolase/hydrolase inhibitor

PDB id: 4pf7

Contents

Protein chains

955 a.a.

Ligands

2QW ×2

Metals

_ZN ×2

Waters ×578

PDB id:

4pf7

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of insulin degrading enzyme complexed with inhibitor

Structure:

Insulin-degrading enzyme. Chain: a, b. Fragment: unp residues 42-1019. Synonym: abeta-degrading protease,insulin protease,insulinase, insulysin. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ide. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.33Å R-factor: 0.199 R-free: 0.236

Authors:

Y.Wang,S.Guo

Key ref:

T.B.Durham et al. (2015). Dual Exosite-binding Inhibitors of Insulin-degrading Enzyme Challenge Its Role as the Primary Mediator of Insulin Clearance in Vivo. J Biol Chem, 290, 20044-20059. PubMed id: 26085101 DOI: 10.1074/jbc.M115.638205

Date:

28-Apr-14 Release date: 17-Jun-15

Protein chains

P14735  (IDE_HUMAN) -  Insulin-degrading enzyme from Homo sapiens

Seq: 1019 a.a.

Struc: 955 a.a.*

* PDB and UniProt seqs differ at 12 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.24.56 - insulysin.
• Reaction: Degradation of insulin, glucagon and other polypeptides. No action on proteins.
• Cofactor: Zn(2+)

DOI no: 10.1074/jbc.M115.638205

J Biol Chem 290:20044-20059 (2015)

PubMed id: 26085101

Dual Exosite-binding Inhibitors of Insulin-degrading Enzyme Challenge Its Role as the Primary Mediator of Insulin Clearance in Vivo.

T.B.Durham, J.L.Toth, V.J.Klimkowski, J.X.Cao, A.M.Siesky, J.Alexander-Chacko, G.Y.Wu, J.T.Dixon, J.E.McGee, Y.Wang, S.Y.Guo, R.N.Cavitt, J.Schindler, S.J.Thibodeaux, N.A.Calvert, M.J.Coghlan, D.K.Sindelar, M.Christe, V.V.Kiselyov, M.D.Michael, K.W.Sloop.

ABSTRACT

No abstract given.