PDBsum entry 4l4v

Membrane protein/immune system

PDB id: 4l4v

Contents

Protein chains

262 a.a.

99 a.a.

200 a.a.

243 a.a.

188 a.a.

Ligands

1VY ×2

GOL ×3

Waters ×1102

PDB id:

4l4v

Name:

Membrane protein/immune system

Title:

Structure of human mait tcr in complex with human mr1-rl-6-me-7-oh

Structure:

Major histocompatibility complex class i-related gene protein. Chain: a, c. Fragment: extracellular domain, residues 23-292. Engineered: yes. Mutation: yes. Beta-2-microglobulin. Chain: b, f. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mr1. Expressed in: escherichia coli. Expression_system_taxid: 511693. Gene: b2m. Gene: tcr alpha. Gene: tcr beta.

Resolution:

1.90Å R-factor: 0.179 R-free: 0.209

Authors:

O.Patel,L.Kjer-Nielsen,J.Le Nours,S.B.G.Eckle,R.W.Birkinshaw, T.Beddoe,A.J.Corbett,L.Liu,J.J.Miles,B.Meehan,R.Reantragoon, M.L.Sandoval-Romero,L.C.Sullivan,A.G.Brooks,Z.Chen,D.P.Fairlie, J.Mccluskey,J.Rossjohn

Key ref:

O.Patel et al. (2013). Recognition of vitamin B metabolites by mucosal-associated invariant T cells. Nat Commun, 4, 2142. PubMed id: 23846752 DOI: 10.1038/ncomms3142

Date:

09-Jun-13 Release date: 17-Jul-13

Protein chains

Q95460  (HMR1_HUMAN) -  Major histocompatibility complex class I-related gene protein from Homo sapiens

Seq: 341 a.a.

Struc: 262 a.a.*

Protein chains

P61769  (B2MG_HUMAN) -  Beta-2-microglobulin from Homo sapiens

Seq: 119 a.a.

Struc: 99 a.a.

Protein chain

Q6P4G7  (Q6P4G7_HUMAN) -  TRA@ protein from Homo sapiens

Seq: 260 a.a.

Struc: 200 a.a.*

Protein chains

P01850  (TRBC1_HUMAN) -  T cell receptor beta constant 1 from Homo sapiens

Seq: 176 a.a.

Struc: 243 a.a.*

Protein chain

Q6P4G7  (Q6P4G7_HUMAN) -  TRA@ protein from Homo sapiens

Seq: 260 a.a.

Struc: 188 a.a.*

* PDB and UniProt seqs differ at 36 residue positions (black crosses)

DOI no: 10.1038/ncomms3142

Nat Commun 4:2142 (2013)

PubMed id: 23846752

Recognition of vitamin B metabolites by mucosal-associated invariant T cells.

O.Patel, L.Kjer-Nielsen, J.Le Nours, S.B.Eckle, R.Birkinshaw, T.Beddoe, A.J.Corbett, L.Liu, J.J.Miles, B.Meehan, R.Reantragoon, M.L.Sandoval-Romero, L.C.Sullivan, A.G.Brooks, Z.Chen, D.P.Fairlie, J.McCluskey, J.Rossjohn.

ABSTRACT

The mucosal-associated invariant T-cell antigen receptor (MAIT TCR) recognizes MR1 presenting vitamin B metabolites. Here we describe the structures of a human MAIT TCR in complex with human MR1 presenting a non-stimulatory ligand derived from folic acid and an agonist ligand derived from a riboflavin metabolite. For both vitamin B antigens, the MAIT TCR docks in a conserved manner above MR1, thus acting as an innate-like pattern recognition receptor. The invariant MAIT TCR α-chain usage is attributable to MR1-mediated interactions that prise open the MR1 cleft to allow contact with the vitamin B metabolite. Although the non-stimulatory antigen does not contact the MAIT TCR, the stimulatory antigen does. This results in a higher affinity of the MAIT TCR for a stimulatory antigen in comparison with a non-stimulatory antigen. We formally demonstrate a structural basis for MAIT TCR recognition of vitamin B metabolites, while illuminating how TCRs recognize microbial metabolic signatures.