PDBsum entry 4bpm

Isomerase

PDB id: 4bpm

Contents

Protein chain

162 a.a.

Ligands

GSH

LVJ

Waters ×37

PDB id:

4bpm

Name:

Isomerase

Title:

Crystal structure of a human integral membrane enzyme

Structure:

Prostaglandin e synthase, fusion peptide. Chain: a. Fragment: gsh-binding motif, residues 10-152. Synonym: microsomal glutathione s-transferase 1-like 1, mgst1-l1, microsomal prostaglandin e synthase 1, mpges-1, p53-induced gene 12 protein, prostaglandin e2 synthase 1. Engineered: yes. Mutation: yes

Source:

Homo sapiens, synthetic construct. Organism_taxid: 9606, 32630. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.

Resolution:

2.08Å R-factor: 0.201 R-free: 0.218

Authors:

D.Li,M.Wang,V.Olieric,M.Caffrey

Key ref:

D.Li et al. (2014). Crystallizing Membrane Proteins in the Lipidic Mesophase. Experience with Human Prostaglandin E2 Synthase 1 and an Evolving Strategy. Cryst Growth Des, 14, 2034-2047. PubMed id: 24803849 DOI: 10.1021/cg500157x

Date:

27-May-13 Release date: 16-Apr-14

Protein chain

O14684  (PTGES_HUMAN) -  Prostaglandin E synthase from Homo sapiens

Seq: 152 a.a.

Struc: 162 a.a.*

Protein chain

Q16873  (LTC4S_HUMAN) -  Leukotriene C4 synthase from Homo sapiens

Seq: 150 a.a.

Struc: 162 a.a.*

* PDB and UniProt seqs differ at 106 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.1.11.1.- - ?????
• Enzyme class 3: E.C.2.5.1.- - ?????
• Enzyme class 4: E.C.2.5.1.18 - glutathione transferase.
• Reaction: RX + glutathione = an S-substituted glutathione + a halide anion + H⁺

RX (Bound ligand (Het Group name = GSH) corresponds exactly) + glutathione = S-substituted glutathione + halide anion + H(+)

• Enzyme class 5: E.C.4.4.1.20 - leukotriene-C4 synthase.

Pathway:

• Reaction: leukotriene C4 = leukotriene A4 + glutathione

leukotriene C4 = leukotriene A4 (Bound ligand (Het Group name = GSH) corresponds exactly) + glutathione

• Enzyme class 6: E.C.5.3.99.3 - prostaglandin-E synthase.
• Reaction: prostaglandin H2 = prostaglandin E2
• Cofactor: Glutathione

Glutathione (Bound ligand (Het Group name = GSH) corresponds exactly)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1021/cg500157x

Cryst Growth Des 14:2034-2047 (2014)

PubMed id: 24803849

Crystallizing Membrane Proteins in the Lipidic Mesophase. Experience with Human Prostaglandin E2 Synthase 1 and an Evolving Strategy.

D.Li, N.Howe, A.Dukkipati, S.T.Shah, B.D.Bax, C.Edge, A.Bridges, P.Hardwicke, O.M.Singh, G.Giblin, A.Pautsch, R.Pfau, G.Schnapp, M.Wang, V.Olieric, M.Caffrey.

ABSTRACT

No abstract given.