PDBsum entry 4hcb

Hydrolase/DNA

PDB id: 4hcb

Contents

Protein chains

462 a.a.

DNA/RNA

Ligands

GOL ×3

SO4 ×8

Waters ×496

PDB id:

4hcb

Name:

Hydrolase/DNA

Title:

The metal-free form of crystal structure of e.Coli exoi-ssdna complex

Structure:

Exodeoxyribonuclease i. Chain: a, b. Synonym: exonuclease i, DNA deoxyribophosphodiesterase, drpase. Engineered: yes. DNA (5'-d( Ap Ap Ap Ap Ap Ap Ap Ap Ap Ap Ap A)-3'). Chain: c, d. Engineered: yes

Source:

Escherichia coli. Organism_taxid: 668369. Strain: dh5alpha. Gene: b2011, cpea, jw1993, sbcb, xona. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: the ssdna samples were synthesized with 3'-phosphoryl modification (page purified)

Resolution:

2.00Å R-factor: 0.185 R-free: 0.224

Authors:

R.Qiu,J.Wei,T.Lou,M.Liu,C.Ji,W.Gong

Key ref:

R.Qiu et al. The structures of escherichia coli exonuclease i in c with the single strand DNA. To be published, .

Date:

29-Sep-12 Release date: 09-Oct-13

Protein chains

P04995  (EX1_ECOLI) -  Exodeoxyribonuclease I from Escherichia coli (strain K12)

Seq: 475 a.a.

Struc: 462 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-A-A-A-A-A-A-A-A-A-A-A 12 bases

A-A-A-A-A-A-A-A-A-A-A-A 12 bases

Enzyme reactions

• Enzyme class: E.C.3.1.11.1 - exodeoxyribonuclease I.
• Reaction: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.