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PDBsum entry 3vdb
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PDB id:
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Hydrolase
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Title:
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E. Coli (lacz) beta-galactosidase (n460t) in complex with galactonolactone
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Structure:
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Beta-galactosidase. Chain: a, b, c, d. Synonym: beta-gal, lactase. Engineered: yes. Mutation: yes
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Source:
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Escherichia coli. Organism_taxid: 562. Gene: lacz. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.05Å
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R-factor:
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0.157
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R-free:
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0.205
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Authors:
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R.W.Wheatley,J.C.Kappelhoff,J.N.Hahn,M.L.Dugdale,M.J.Dutkoski, S.D.Tamman,M.E.Fraser,R.E.Huber
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Key ref:
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R.W.Wheatley
et al.
(2012).
Substitution for Asn460 cripples β-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability.
Arch Biochem Biophys,
521,
51-61.
PubMed id:
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Date:
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04-Jan-12
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Release date:
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11-Apr-12
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PROCHECK
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Headers
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References
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P00722
(BGAL_ECOLI) -
Beta-galactosidase from Escherichia coli (strain K12)
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Seq:
Struc:
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1024 a.a.
1015 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.3.2.1.23
- beta-galactosidase.
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Reaction:
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Hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.
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Arch Biochem Biophys
521:51-61
(2012)
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PubMed id:
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Substitution for Asn460 cripples β-galactosidase (Escherichia coli) by increasing substrate affinity and decreasing transition state stability.
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R.W.Wheatley,
J.C.Kappelhoff,
J.N.Hahn,
M.L.Dugdale,
M.J.Dutkoski,
S.D.Tamman,
M.E.Fraser,
R.E.Huber.
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ABSTRACT
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Links
