PDBsum entry 3mv0

Hydrolase

PDB id: 3mv0

Contents

Protein chains

1011 a.a.

Ligands

149 ×4

DMS ×122

Metals

_MG ×11

_NA ×16

Waters ×3151

PDB id:

3mv0

Name:

Hydrolase

Title:

E. Coli (lacz) beta-galactosidase (r599a) in complex with d- galctopyranosyl-1-one

Structure:

Beta-galactosidase. Chain: 1, 2, 3, 4. Engineered: yes. Mutation: yes

Source:

Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: lacz. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.20Å R-factor: 0.195 R-free: 0.237

Authors:

M.L.Dugdale,M.Vance,M.L.Driedger,A.Nibber,A.Tran,R.E.Huber

Key ref:

M.L.Dugdale et al. (2010). Importance of arg-599 of b-Galactosidase (escherichia as an anchor for the open conformations of phe-601 an active-Site loop. Biochem cell biol, 88, 969.

Date:

03-May-10 Release date: 16-Mar-11

Protein chains

P00722  (BGAL_ECOLI) -  Beta-galactosidase from Escherichia coli (strain K12)

Seq: 1024 a.a.

Struc: 1011 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.2.1.23 - beta-galactosidase.
• Reaction: Hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-D-galactosides.