PDBsum entry 3j8c

Translation

PDB id: 3j8c

Contents

Protein chains

488 a.a.

546 a.a.

384 a.a.

232 a.a.

254 a.a.

204 a.a.

258 a.a.

176 a.a.

PDB id:

3j8c

Name:

Translation

Title:

Model of the human eif3 pci-mpn octamer docked into the 43s em map

Structure:

Eukaryotic translation initiation factor 3 subunit a. Chain: a. Fragment: see remark 999. Synonym: eif3a, eukaryotic translation initiation factor 3 subunit 10, eif-3-theta, eif3 p167, eif3 p180, eif3 p185. Eukaryotic translation initiation factor 3 subunit c. Chain: c. Fragment: see remark 999. Synonym: eif3c, eukaryotic translation initiation factor 3 subunit 8,

Source:

Homo sapiens. Human. Organism_taxid: 9606. Organism_taxid: 9606

Authors:

J.P.Erzberger,N.Ban

Key ref:

J.P.Erzberger et al. (2014). Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex. Cell, 158, 1123-1135. PubMed id: 25171412 DOI: 10.1016/j.cell.2014.07.044

Date:

08-Oct-14 Release date: 22-Oct-14

Supersedes:

3j7k

Protein chain

Q14152  (EIF3A_HUMAN) -  Eukaryotic translation initiation factor 3 subunit A from Homo sapiens

Seq: 1382 a.a.

Struc: 488 a.a.*

Protein chain

Q99613  (EIF3C_HUMAN) -  Eukaryotic translation initiation factor 3 subunit C from Homo sapiens

Seq: 913 a.a.

Struc: 546 a.a.*

Protein chain

P60228  (EIF3E_HUMAN) -  Eukaryotic translation initiation factor 3 subunit E from Homo sapiens

Seq: 445 a.a.

Struc: 384 a.a.*

Protein chain

O00303  (EIF3F_HUMAN) -  Eukaryotic translation initiation factor 3 subunit F from Homo sapiens

Seq: 357 a.a.

Struc: 232 a.a.*

Protein chain

O15372  (EIF3H_HUMAN) -  Eukaryotic translation initiation factor 3 subunit H from Homo sapiens

Seq: 352 a.a.

Struc: 254 a.a.*

Protein chain

Q9UBQ5  (EIF3K_HUMAN) -  Eukaryotic translation initiation factor 3 subunit K from Homo sapiens

Seq: 218 a.a.

Struc: 204 a.a.*

Protein chain

Q9Y262  (EIF3L_HUMAN) -  Eukaryotic translation initiation factor 3 subunit L from Homo sapiens

Seq: 564 a.a.

Struc: 258 a.a.*

Protein chain

Q7L2H7  (EIF3M_HUMAN) -  Eukaryotic translation initiation factor 3 subunit M from Homo sapiens

Seq: 374 a.a.

Struc: 176 a.a.*

* PDB and UniProt seqs differ at 308 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain F: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

DOI no: 10.1016/j.cell.2014.07.044

Cell 158:1123-1135 (2014)

PubMed id: 25171412

Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex.

J.P.Erzberger, F.Stengel, R.Pellarin, S.Zhang, T.Schaefer, C.H.Aylett, P.Cimermančič, D.Boehringer, A.Sali, R.Aebersold, N.Ban.

ABSTRACT

Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core. These structures, together with electron microscopy reconstructions, cross-linking coupled to mass spectrometry, and integrative structure modeling, allowed us to position and orient all eIF3 components on the 40S⋅eIF1 complex, revealing an extended, modular arrangement of eIF3 subunits. Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery. The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA.