PDBsum entry 3v4x

Transferase/inhibitor

PDB id: 3v4x

Contents

Protein chains

387 a.a.

Ligands

F24 ×4

Waters ×1087

PDB id:

3v4x

Name:

Transferase/inhibitor

Title:

The biochemical and structural basis for inhibition of enterococcus faecalis hmg-coa synthase, mvas, by hymeglusin

Structure:

Hmg-coa synthase. Chain: a, b, c, d. Engineered: yes. Mutation: yes

Source:

Enterococcus faecalis. Organism_taxid: 1351. Strain: atcc 4200. Gene: mvas. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.95Å R-factor: 0.176 R-free: 0.218

Authors:

D.A.Skaff,K.X.Ramyar,W.J.Mcwhorter,B.V.Geisbrecht,H.M.Miziorko

Key ref:

D.A.Skaff et al. (2012). Biochemical and structural basis for inhibition of Enterococcus faecalis hydroxymethylglutaryl-CoA synthase, mvaS, by hymeglusin. Biochemistry, 51, 4713-4722. PubMed id: 22510038

Date:

15-Dec-11 Release date: 25-Apr-12

Protein chains

Q9FD71  (HMGCS_ENTFL) -  Hydroxymethylglutaryl-CoA synthase from Enterococcus faecalis

Seq: 383 a.a.

Struc: 387 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.2.3.3.10 - hydroxymethylglutaryl-CoA synthase.
• Pathway: Mevalonate Biosynthesis
• Reaction: acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Biochemistry 51:4713-4722 (2012)

PubMed id: 22510038

Biochemical and structural basis for inhibition of Enterococcus faecalis hydroxymethylglutaryl-CoA synthase, mvaS, by hymeglusin.

D.A.Skaff, K.X.Ramyar, W.J.McWhorter, M.L.Barta, B.V.Geisbrecht, H.M.Miziorko.

ABSTRACT

No abstract given.