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UniProt functional annotation for Q9FD71 | ||
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| UniProt code: Q9FD71. |
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| Organism: | |
Enterococcus faecalis (Streptococcus faecalis). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus. |
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| Function: | |
Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds. {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}. |
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| Catalytic activity: | |
Reaction=acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-hydroxy-3- methylglutaryl-CoA + CoA + H(+); Xref=Rhea:RHEA:10188, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43074, ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.3.10; Evidence={ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}; |
| Activity regulation: | |
Is sensitive to feedback substrate inhibition by acetoacetyl-CoA. Is inactivated by hymeglusin, which also blocks the growth of E.faecalis, indicating the critical role that the mevalonate pathway plays in isoprenoid biosynthesis. {ECO:0000269|PubMed:16245942, ECO:0000269|PubMed:22510038, ECO:0000269|PubMed:23794621}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=350 uM for acetyl-CoA {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}; KM=10 uM for acetoacetyl-CoA {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}; KM=400 uM for acetyl-CoA {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}; KM=0.5 uM for acetoacetyl-CoA {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}; Vmax=10 umol/min/mg enzyme {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}; Vmax=5.3 umol/min/mg enzyme {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:23794621}; pH dependence: Optimum pH is about 9.8. {ECO:0000269|PubMed:12107122}; Temperature dependence: Optimum temperature is 37 degrees Celsius. {ECO:0000269|PubMed:12107122}; |
| Pathway: | |
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3. {ECO:0000269|PubMed:10894743}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:12107122, ECO:0000269|PubMed:16245942, ECO:0000269|PubMed:22510038}. |
| Similarity: | |
Belongs to the thiolase-like superfamily. HMG-CoA synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.