PDBsum entry 3jc6

Replication

PDB id: 3jc6

Contents

Protein chains

241 a.a.

269 a.a.

275 a.a.

254 a.a.

267 a.a.

325 a.a.

553 a.a.

221 a.a.

181 a.a.

208 a.a.

159 a.a.

Metals

_ZN

PDB id:

3jc6

Name:

Replication

Title:

Structure of the eukaryotic replicative cmg helicase and pumpjack motion

Structure:

DNA replication licensing factor mcm2. Chain: 2. Synonym: minichromosome maintenance protein 2. Engineered: yes. DNA replication licensing factor mcm3. Chain: 3. Synonym: minichromosome maintenance protein 3. Engineered: yes. DNA replication licensing factor mcm4.

Source:

Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Gene: mcm2, ybl023c, ybl0438. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932. Gene: mcm3, yel032w, sygp-orf23. Gene: mcm4, cdc54, hcd21, ypr019w, yp9531.13. Gene: mcm5, cdc46, ylr274w, l9328.1.

Authors:

H.Li,L.Bai,Z.Yuan,J.Sun,R.E.Georgescu,J.Liu,M.E.O'Donnell

Key ref:

Z.Yuan et al. (2016). Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation. Nat Struct Biol, 23, 217-224. PubMed id: 26854665 DOI: 10.1038/nsmb.3170

Date:

24-Nov-15 Release date: 10-Feb-16

Protein chain

P29469  (MCM2_YEAST) -  DNA replication licensing factor MCM2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 868 a.a.

Struc: 241 a.a.

Protein chain

P24279  (MCM3_YEAST) -  DNA replication licensing factor MCM3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 971 a.a.

Struc: 269 a.a.

Protein chain

P30665  (MCM4_YEAST) -  DNA replication licensing factor MCM4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 933 a.a.

Struc: 275 a.a.

Protein chain

P29496  (MCM5_YEAST) -  Minichromosome maintenance protein 5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 775 a.a.

Struc: 254 a.a.

Protein chain

P53091  (MCM6_YEAST) -  DNA replication licensing factor MCM6 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1017 a.a.

Struc: 267 a.a.

Protein chain

P38132  (MCM7_YEAST) -  DNA replication licensing factor MCM7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 845 a.a.

Struc: 325 a.a.

Protein chain

Q08032  (CDC45_YEAST) -  Cell division control protein 45 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 650 a.a.

Struc: 553 a.a.

Protein chain

Q03406  (SLD5_YEAST) -  DNA replication complex GINS protein SLD5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 294 a.a.

Struc: 221 a.a.

Protein chain

P40359  (PSF2_YEAST) -  DNA replication complex GINS protein PSF2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 213 a.a.

Struc: 181 a.a.

Protein chain

Q12488  (PSF1_YEAST) -  DNA replication complex GINS protein PSF1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 208 a.a.

Struc: 208 a.a.

Protein chain

Q12146  (PSF3_YEAST) -  DNA replication complex GINS protein PSF3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 194 a.a.

Struc: 159 a.a.

Enzyme reactions

• Enzyme class: Chains 2, 3, 4, 5, 6, 7: E.C.3.6.4.12 - Dna helicase.
• Reaction: ATP + H2O = ADP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1038/nsmb.3170

Nat Struct Biol 23:217-224 (2016)

PubMed id: 26854665

Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation.

Z.Yuan, L.Bai, J.Sun, R.Georgescu, J.Liu, M.E.O'Donnell, H.Li.

ABSTRACT

The CMG helicase is composed of Cdc45, Mcm2-7 and GINS. Here we report the structure of the Saccharomyces cerevisiae CMG, determined by cryo-EM at a resolution of 3.7-4.8 Å. The structure reveals that GINS and Cdc45 scaffold the N tier of the helicase while enabling motion of the AAA+ C tier. CMG exists in two alternating conformations, compact and extended, thus suggesting that the helicase moves like an inchworm. The N-terminal regions of Mcm2-7, braced by Cdc45-GINS, form a rigid platform upon which the AAA+ C domains make longitudinal motions, nodding up and down like an oil-rig pumpjack attached to a stable platform. The Mcm ring is remodeled in CMG relative to the inactive Mcm2-7 double hexamer. The Mcm5 winged-helix domain is inserted into the central channel, thus blocking entry of double-stranded DNA and supporting a steric-exclusion DNA-unwinding model.