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PDBsum entry 2yii
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PDB id:
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Lyase
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Title:
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Manipulating the regioselectivity of phenylalanine aminomutase: new insights into the reaction mechanism of mio-dependent enzymes from structure-guided directed evolution
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Structure:
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Phenylalanine ammonia-lyase. Chain: a, b, c, d. Synonym: phenylalanine aminomutase. Engineered: yes. Other_details: thioester bond between the mdo prosthetic group and a molecule of beta-mercaptoethanol, disulfide bridge between residue c89 and a beta-mercaptoethanol molecule, covalent bond between mdo 175 and residue d178
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Source:
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Taxus wallichiana var. Chinensis. Chinese yew. Organism_taxid: 29808. Expressed in: escherichia coli. Expression_system_taxid: 83333. Expression_system_variant: top10.
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Resolution:
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2.18Å
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R-factor:
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0.181
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R-free:
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0.215
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Authors:
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B.Wu,W.Szymanski,G.G.Wybenga,M.M.Heberling,S.Bartsch,S.Wildeman, G.J.Poelarends,B.L.Feringa,B.W.Dijkstra,D.B.Janssen
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Key ref:
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B.Wu
et al.
(2012).
Mechanism-inspired engineering of phenylalanine aminomutase for enhanced β-regioselective asymmetric amination of cinnamates.
Angew Chem Int Ed Engl,
51,
482-486.
PubMed id:
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Date:
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13-May-11
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Release date:
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09-Nov-11
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PROCHECK
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Headers
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References
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Q68G84
(PAM_TAXCH) -
Phenylalanine aminomutase (L-beta-phenylalanine forming) from Taxus chinensis
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Seq:
Struc:
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687 a.a.
644 a.a.*
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Key: |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class 2:
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E.C.4.3.1.24
- phenylalanine ammonia-lyase.
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Reaction:
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L-phenylalanine = (E)-cinnamate + NH4+
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L-phenylalanine
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=
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(E)-cinnamate
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+
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NH4(+)
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Cofactor:
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MIO
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Enzyme class 3:
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E.C.5.4.3.10
- phenylalanine aminomutase (L-beta-phenylalanine forming).
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Reaction:
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L-phenylalanine = L-beta-phenylalanine
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L-phenylalanine
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=
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L-beta-phenylalanine
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Cofactor:
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MIO
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Note, where more than one E.C. class is given (as above), each may
correspond to a different protein domain or, in the case of polyprotein
precursors, to a different mature protein.
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Angew Chem Int Ed Engl
51:482-486
(2012)
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PubMed id:
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Mechanism-inspired engineering of phenylalanine aminomutase for enhanced β-regioselective asymmetric amination of cinnamates.
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B.Wu,
W.Szymański,
G.G.Wybenga,
M.M.Heberling,
S.Bartsch,
S.de Wildeman,
G.J.Poelarends,
B.L.Feringa,
B.W.Dijkstra,
D.B.Janssen.
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ABSTRACT
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Links
