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UniProt functional annotation for Q68G84 | ||
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| UniProt code: Q68G84. |
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| Organism: | |
Taxus wallichiana var. chinensis (Chinese yew) (Taxus chinensis). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Pinopsida; Pinidae; Cupressales; Taxaceae; Taxus. |
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| Function: | |
Phenylalanine aminomutase that catalyzes the rearrangement of L-phenylalanine to R-beta-phenylalanine. Catalyzes the first committed step in the biosynthesis of the side chain of the alkaloid taxol (paclitaxel), a widely-used compound with antitumor activity. Has also low phenylalanine ammonia-lyase activity and can catalyze the amination of trans-cinnamate. {ECO:0000269|PubMed:15878763, ECO:0000269|PubMed:22113970, ECO:0000269|PubMed:24786474}. |
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| Catalytic activity: | |
Reaction=L-phenylalanine = L-beta-phenylalanine; Xref=Rhea:RHEA:34395, ChEBI:CHEBI:58095, ChEBI:CHEBI:67158; EC=5.4.3.10; Evidence={ECO:0000269|PubMed:15878763, ECO:0000269|PubMed:22113970}; |
| Catalytic activity: | |
Reaction=L-phenylalanine = (E)-cinnamate + NH4(+); Xref=Rhea:RHEA:21384, ChEBI:CHEBI:15669, ChEBI:CHEBI:28938, ChEBI:CHEBI:58095; EC=4.3.1.24; Evidence={ECO:0000250|UniProtKB:Q6GZ04}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.1 mM for L-phenylalanine {ECO:0000269|PubMed:15878763, ECO:0000269|PubMed:22113970}; Vmax=110 umol/min/mg enzyme with L-phenylalanine as substrate {ECO:0000269|PubMed:15878763, ECO:0000269|PubMed:22113970}; pH dependence: Optimum pH is 7.5-8.0. {ECO:0000269|PubMed:15878763, ECO:0000269|PubMed:22113970}; |
| Pathway: | |
Alkaloid biosynthesis; taxol biosynthesis. |
| Pathway: | |
Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1. |
| Subunit: | |
Homodimer (PubMed:15878763). Homotetramer, dimer of dimers (PubMed:24786474). {ECO:0000269|PubMed:15878763, ECO:0000269|PubMed:24786474}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Ptm: | |
Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly. {ECO:0000269|PubMed:24786474}. |
| Biotechnology: | |
Could be used for the stereoselective biosynthesis of beta-amino acids via amination of cinnamic acid derivatives. {ECO:0000269|PubMed:22113970}. |
| Similarity: | |
Belongs to the PAL/histidase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.