PDBsum entry 1z8c

Hydrolase/hydrolase inhibitor

PDB id: 1z8c

Contents

Protein chains

99 a.a. *

Ligands

0ZS

SO4

Waters ×110

* Residue conservation analysis

PDB id:

1z8c

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of the complex of mutant HIV-1 protease (l63p, a71v, v82t, i84v) with a hydroxyethylamine peptidomimetic inhibitor boc- phe-psi[r-ch(oh)ch2nh]-phe-glu-phe-nh2

Structure:

Protease retropepsin. Chain: a, b. Synonym: HIV-1 protease. Engineered: yes. Mutation: yes

Source:

Human immunodeficiency virus 1. Organism_taxid: 11676. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Biol. unit:

Trimer (from PQS)

Resolution:

2.20Å R-factor: 0.189 R-free: 0.257

Authors:

J.Duskova,T.Skalova,J.Dohnalek,H.Petrokova,J.Hasek

Key ref:

J.Duskova et al. Mutational study of pseudopeptide inhibitor binding to HIV-1 protease. To be published, .

Date:

30-Mar-05 Release date: 21-Mar-06

Protein chains

Q9JAU9  (Q9JAU9_HV1) -  Protease (Fragment) from Human immunodeficiency virus type 1

Seq: 99 a.a.

Struc: 99 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: E.C.3.4.23.16 - HIV-1 retropepsin.
• Reaction: Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
• Enzyme class 3: E.C.2.7.7.- - ?????
• Enzyme class 4: E.C.2.7.7.49 - RNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 5: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 6: E.C.3.1.-.-
• Enzyme class 7: E.C.3.1.13.2 - exoribonuclease H.
• Reaction: Exonucleolytic cleavage to 5'-phosphomonoester oligonucleotides in both 5'- to 3'- and 3'- to 5'-directions.
• Enzyme class 8: E.C.3.1.26.13 - retroviral ribonuclease H.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site