PDBsum entry 1xqg

Antitumor protein

PDB id: 1xqg

Contents

Protein chain

378 a.a. *

Waters ×152

* Residue conservation analysis

PDB id:

1xqg

Name:

Antitumor protein

Title:

3.10 a crystal structure of maspin, space group p 4 21 2

Structure:

Maspin. Chain: a, b. Engineered: yes. Mutation: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: serpinb5, pi5. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.10Å R-factor: 0.194 R-free: 0.252

Authors:

M.Al-Ayyoubi,P.G.Gettins,K.Volz

Key ref:

M.Al-Ayyoubi et al. (2004). Crystal structure of human maspin, a serpin with antitumor properties: reactive center loop of maspin is exposed but constrained. J Biol Chem, 279, 55540-55544. PubMed id: 15501821 DOI: 10.1074/jbc.M409957200

Date:

12-Oct-04 Release date: 26-Oct-04

Protein chains

P36952  (SPB5_HUMAN) -  Serpin B5 from Homo sapiens

Seq: 375 a.a.

Struc: 378 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

DOI no: 10.1074/jbc.M409957200

J Biol Chem 279:55540-55544 (2004)

PubMed id: 15501821

Crystal structure of human maspin, a serpin with antitumor properties: reactive center loop of maspin is exposed but constrained.

M.Al-Ayyoubi, P.G.Gettins, K.Volz.

ABSTRACT

Maspin, a member of the serpin superfamily, has tumor suppressing activity against breast and prostate cancer. Maspin inhibits tumor growth by blocking cell invasion, and its reactive center loop (RCL) is thought to mediate this activity. To understand this function on the molecular level, we have solved the three-dimensional structure of Maspin to 3.1 A resolution. The molecular structure shows the characteristic features of the serpin fold, but the RCL of Maspin is unique in length, composition, and placement. Although the RCL of Maspin is accessible and cleavable by some proteinases, it functions in the uncleaved, constrained conformation observed here. These structural results will contribute to our understanding of the mechanism by which Maspin suppresses tumors.

Selected figure(s)

Figure 2.
FIG. 2. Stereo view of Maspin ribbon diagram in the typical serpin orientation. -Strands are cyan, -helices are magenta, and coils and turns are gold. -Sheets and helices are labeled as in Fig. 1.

Figure 3.
FIG. 3. Stereo diagram of the |F[o] - F[c]| omit map of residues 330-346 of the RCL of Maspin, contoured at 2 . View is from the back right of Fig. 2.

The above figures are reprinted by permission from the ASBMB: J Biol Chem (2004, 279, 55540-55544) copyright 2004.

Figures were selected by an automated process.