 |
PDBsum entry 1xqg
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Antitumor protein
|
PDB id
|
|
|
|
1xqg
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Crystal structure of human maspin, A serpin with antitumor properties: reactive center loop of maspin is exposed but constrained.
|
|
|
Authors
|
|
M.Al-Ayyoubi,
P.G.Gettins,
K.Volz.
|
|
|
Ref.
|
|
J Biol Chem, 2004,
279,
55540-55544.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Maspin, a member of the serpin superfamily, has tumor suppressing activity
against breast and prostate cancer. Maspin inhibits tumor growth by blocking
cell invasion, and its reactive center loop (RCL) is thought to mediate this
activity. To understand this function on the molecular level, we have solved the
three-dimensional structure of Maspin to 3.1 A resolution. The molecular
structure shows the characteristic features of the serpin fold, but the RCL of
Maspin is unique in length, composition, and placement. Although the RCL of
Maspin is accessible and cleavable by some proteinases, it functions in the
uncleaved, constrained conformation observed here. These structural results will
contribute to our understanding of the mechanism by which Maspin suppresses
tumors.
|
|
|
|
|
|
|
|
Figure 2.
FIG. 2. Stereo view of Maspin ribbon diagram in the typical
serpin orientation.  -Strands are cyan,  -helices
are magenta, and coils and turns are gold. -Sheets and helices are
labeled as in Fig. 1.
|
|
Figure 3.
FIG. 3. Stereo diagram of the |F[o] - F[c]| omit map of
residues 330-346 of the RCL of Maspin, contoured at 2  . View
is from the back right of Fig. 2.
|
|
|
|
|
|
The above figures are
reprinted
by permission from the ASBMB:
J Biol Chem
(2004,
279,
55540-55544)
copyright 2004.
|
|
|
|
|
|
|
