8ea4

Electron Microscopy
3Å resolution

V-K CAST Transpososome from Scytonema hofmanni, minor configuration

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
PDBe Complex ID:
PDB-CPX-221070 (preferred)
Entry contents:
5 distinct polypeptide molecules
6 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (12 distinct):
Transposase Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M
Length: 276 amino acids
Theoretical weight: 31.44 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A8J0PCL3 (Residues: 1-276; Coverage: 100%)
Sequence domains: Bacterial TniB protein
Cas12k Chain: O
Molecule details ›
Chain: O
Length: 639 amino acids
Theoretical weight: 73.28 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A8M0FGU0 (Residues: 1-639; Coverage: 100%)
Transcriptional regulator Chain: Q
Molecule details ›
Chain: Q
Length: 167 amino acids
Theoretical weight: 19.01 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A8J0PCL5 (Residues: 1-167; Coverage: 100%)
Sequence domains: TniQ
Small ribosomal subunit protein uS15 Chain: S
Molecule details ›
Chain: S
Length: 89 amino acids
Theoretical weight: 10.29 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ADZ4 (Residues: 1-89; Coverage: 100%)
Gene names: JW3134, b3165, rpsO, secC
Sequence domains: Ribosomal protein S15
Integrase catalytic domain-containing protein Chains: W, X, Y, Z, w, x, y, z
Molecule details ›
Chains: W, X, Y, Z, w, x, y, z
Length: 584 amino acids
Theoretical weight: 66.64 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A979HMQ2 (Residues: 1-584; Coverage: 100%)
Sequence domains:
Target-LE Chain: 1
Molecule details ›
Chain: 1
Length: 141 nucleotides
Theoretical weight: 43.44 KDa
LE_R Chain: 2
Molecule details ›
Chain: 2
Length: 51 nucleotides
Theoretical weight: 15.78 KDa
Non-target_R Chain: 3
Molecule details ›
Chain: 3
Length: 71 nucleotides
Theoretical weight: 21.81 KDa
RE_F Chain: 4
Molecule details ›
Chain: 4
Length: 75 nucleotides
Theoretical weight: 23.08 KDa
RE_R1 Chain: 5
Molecule details ›
Chain: 5
Length: 50 nucleotides
Theoretical weight: 15.44 KDa
RE_R2 Chain: 6
Molecule details ›
Chain: 6
Length: 20 nucleotides
Theoretical weight: 6.09 KDa
sg_RNA Chain: 7
Molecule details ›
Chain: 7
Length: 265 nucleotides
Theoretical weight: 85.26 KDa

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-27972
Expression system: Escherichia coli