› 8bie

X-ray diffraction
2.25Å resolution

O-Methyltransferase Plu4894 in complex with SAH

Released:
DOI: 10.2210/pdb8bie/pdb
PDB entry 8bie coloured by chain, front view.
PDB entry 8bie coloured by chain, side view.
PDB entry 8bie coloured by chain, top view.
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Primary publication:
A set of closely related methyltransferases for site-specific tailoring of anthraquinone pigments.
Huber EM, Kreling L, Heinrich AK, Dünnebacke M, Pöthig A, Bode HB, Groll M
Structure (2023)
PMID: 36963398

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-227929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-methyltransferase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 318 amino acids
Theoretical weight: 36.03 KDa
Source organism: Photorhabdus laumondii subsp. laumondii TTO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7MY05 (Residues: 1-317; Coverage: 100%)
Gene name: plu4894
Sequence domains: O-methyltransferase domain

Ligands and Environments

1 bound ligand:
Ligand SAH 2 x SAH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 44.91Å b: 79.95Å c: 96.51Å
α: 90° β: 99.94° γ: 90°
R-values:
R R work R free
0.199 0.197 0.228
Expression system: Escherichia coli BL21(DE3)

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