› 8f2p

X-ray diffraction
2.63Å resolution

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136960 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
C-terminal core protein Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 17.59 KDa
Source organism: HIV-1 M:B_ARV2/SF2
Expression system: Escherichia coli
UniProt:
  • Canonical: P03407 (Residues: 62-209; Coverage: 71%)
Gene name: nef
Sequence domains: Negative factor, (F-Protein) or Nef
Tyrosine-protein kinase HCK Chain: B
Molecule details ›
Chain: B
Length: 73 amino acids
Theoretical weight: 8.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08631 (Residues: 77-140; Coverage: 12%)
Gene name: HCK
Sequence domains: SH3 domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P41212
Unit cell:
a: 84.051Å b: 84.051Å c: 83.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.252
Expression system: Escherichia coli

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