› 7xou

Electron Microscopy
3.2Å resolution

Structural insights into human brain gut peptide cholecystokinin receptors

Released:
DOI: 10.2210/pdb7xou/pdb
PDB entry 7xou coloured by chain, front view.
PDB entry 7xou coloured by chain, side view.
PDB entry 7xou coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into human brain-gut peptide cholecystokinin receptors.
Ding Y, Zhang H, Liao YY, Chen LN, Ji SY, Qin J, Mao C, Shen DD, Lin L, Wang H, Zhang Y, Li XM
OpenAccess logo Cell Discov 8 55 (2022)
PMID: 35672283
Related structures: EMD-33359

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-225077 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Cholecystokinin-8 Chain: L
Molecule details ›
Chain: L
Length: 9 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P06307 (Residues: 96-103; Coverage: 8%)
Gene name: CCK
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 1-394; Coverage: 96%)
  • Best match: P63092-2 (Residues: 1-380)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 384 amino acids
Theoretical weight: 41.73 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 126 amino acids
Theoretical weight: 13.71 KDa
Source organism: synthetic construct
Expression system: Not provided
Cholecystokinin receptor type A Chain: R
Molecule details ›
Chain: R
Length: 428 amino acids
Theoretical weight: 47.89 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32238 (Residues: 1-428; Coverage: 100%)
Gene names: CCKAR, CCKRA
Sequence domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TYS 1 x TYS

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-33359
Expression systems:
  • Not provided
  • Spodoptera frugiperda

Quick links

Citations

2 citation in other articles

A Novel CCK Receptor GPR173 Mediates Potentiation of GABAergic Inhibition.
He et al. (2023)
1 more