› 7t83

X-ray diffraction
2.1Å resolution

Structure of angiotensin II type I receptor (AT1R) nanobody antagonist AT118i4h32

Released:
DOI: 10.2210/pdb7t83/pdb
PDB entry 7t83 coloured by chain, front view.
PDB entry 7t83 coloured by chain, side view.
PDB entry 7t83 coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
An in silico method to assess antibody fragment polyreactivity.
Harvey EP, Shin JE, Skiba MA, Nemeth GR, Hurley JD, Wellner A, Shaw AY, Miranda VG, Min JK, Liu CC, Marks DS, Kruse AC
Nat Commun 13 7554 (2022)
PMID: 36477674

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233716 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nanobody AT118i4h32 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 128 amino acids
Theoretical weight: 14.23 KDa
Source organism: synthetic construct
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand FLC 4 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 80.11Å b: 85.4Å c: 84.44Å
α: 90° β: 108.64° γ: 90°
R-values:
R R work R free
0.209 0.208 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Accelerating therapeutic protein design with computational approaches toward the clinical stage.
Chen et al. (2023)
1 other article

1 mention without citation

Antibodies Expand the Scope of Angiotensin Receptor Pharmacology.
Skiba et al. (2023)