› 7rzo

X-ray diffraction
1.8Å resolution

Crystal structure of a dihydrofolate reductase (folA) from Stenotrophomonas maltophilia

Released:
DOI: 10.2210/pdb7rzo/pdb
PDB entry 7rzo coloured by chain, front view.
PDB entry 7rzo coloured by chain, side view.
PDB entry 7rzo coloured by chain, top view.
Source organism: Stenotrophomonas maltophilia K279a
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109521 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chain: A
Molecule details ›
Chain: A
Length: 171 amino acids
Theoretical weight: 19.27 KDa
Source organism: Stenotrophomonas maltophilia K279a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2FPG1 (Residues: 1-163; Coverage: 100%)
Gene names: Smlt0814, folA
Sequence domains: Dihydrofolate reductase

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: I4122
Unit cell:
a: 104.17Å b: 104.17Å c: 87.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.157 0.187
Expression system: Escherichia coli BL21(DE3)

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