7qd6

Electron Microscopy
3Å resolution

Cryo-EM structure of Tn4430 TnpA transposase from Tn3 family in complex with strand-transfer like DNA product

Released:
Source organism: Bacillus thuringiensis
Related structures: EMD-13909

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-121853 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Transposase for transposon Tn4430 Chains: A, D
Molecule details ›
Chains: A, D
Length: 1014 amino acids
Theoretical weight: 116.99 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli
UniProt:
  • Canonical: P10021 (Residues: 1-987; Coverage: 100%)
Gene name: tnpA
Sequence domains:
IR71st non transferred strand Chains: B, E
Molecule details ›
Chains: B, E
Length: 71 nucleotides
Theoretical weight: 21.86 KDa
Source organism: Bacillus thuringiensis
Expression system: Not provided
IR71st transferred strand Chains: C, F
Molecule details ›
Chains: C, F
Length: 78 nucleotides
Theoretical weight: 24.13 KDa
Source organism: Bacillus thuringiensis
Expression system: Not provided
IR71st transferred strand Chains: G, H
Molecule details ›
Chains: G, H
Length: 23 nucleotides
Theoretical weight: 7.11 KDa
Source organism: Bacillus thuringiensis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-13909
Expression systems:
  • Escherichia coli
  • Not provided