› 7k36

Electron Microscopy
3.3Å resolution

Cryo-EM structure of STRIPAK complex

Released:
DOI: 10.2210/pdb7k36/pdb
PDB entry 7k36 coloured by chain, front view.
PDB entry 7k36 coloured by chain, side view.
PDB entry 7k36 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of the Hippo signaling integrator human STRIPAK.
Jeong BC, Bae SJ, Ni L, Zhang X, Bai XC, Luo X
Nat Struct Mol Biol 28 290-299 (2021)
PMID: 33633399
Related structures: EMD-22650

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-151663 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 589 amino acids
Theoretical weight: 65.38 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P30153 (Residues: 1-589; Coverage: 100%)
Gene name: PPP2R1A
Sequence domains: HEAT repeat
Striatin-3 Chains: B, D, E, F, G
Molecule details ›
Chains: B, D, E, F, G
Length: 713 amino acids
Theoretical weight: 77.83 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q13033 (Residues: 1-797; Coverage: 90%)
  • Best match: Q13033-2 (Residues: 1-713)
Gene names: GS2NA, SG2NA, STRN3
Sequence domains:
Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 309 amino acids
Theoretical weight: 35.64 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: P67775 (Residues: 1-309; Coverage: 100%)
Gene name: PPP2CA
Sequence domains: Calcineurin-like phosphoesterase
MOB-like protein phocein Chain: H
Molecule details ›
Chain: H
Length: 225 amino acids
Theoretical weight: 26.06 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q9Y3A3 (Residues: 1-225; Coverage: 100%)
Gene names: CGI-95, MOB3, MOB4, MOBKL3, PHOCN, PREI3
Sequence domains: Mob1/phocein family
Striatin-interacting protein 1 Chain: I
Molecule details ›
Chain: I
Length: 837 amino acids
Theoretical weight: 95.7 KDa
Source organism: Homo sapiens
Expression system: Baculovirus expression vector pFastBac1-HM
UniProt:
  • Canonical: Q5VSL9 (Residues: 1-837; Coverage: 100%)
Gene names: FAM40A, KIAA1761, STRIP1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand ZN 2 x ZN
Ligand IHP 1 x IHP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.3Å
Relevant EMDB volumes: EMD-22650
Expression system: Baculovirus expression vector pFastBac1-HM

Quick links

Citations

5 review citations

Cellular feedback dynamics and multilevel regulation driven by the hippo pathway.
Park et al. (2021)
4 more

5 mentions without citation

Protein phosphatase 2A - structure, function and role in neurodevelopmental disorders.
Sandal et al. (2021)
4 more