7x4n

X-ray diffraction
2.88Å resolution

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-124530 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1A chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.12 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02550 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1A
Sequence domains:
Tubulin beta chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
DARPin Chain: C
Molecule details ›
Chain: C
Length: 159 amino acids
Theoretical weight: 16.79 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Kinesin-like protein klp-12 Chain: E
Molecule details ›
Chain: E
Length: 365 amino acids
Theoretical weight: 40.01 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: G5EGS3 (Residues: 1-365; Coverage: 23%)
Gene names: T01G1.1, klp-12
Sequence domains: Kinesin motor domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL32XU
Spacegroup: P21
Unit cell:
a: 82.33Å b: 80.98Å c: 117.75Å
α: 90° β: 93.51° γ: 90°
R-values:
R R work R free
0.215 0.21 0.296
Expression system: Escherichia coli