› 7w57

Electron Microscopy
3.2Å resolution

Cryo-EM structure of the neuromedin S-bound neuromedin U receptor 2-Gq protein complex

Released:
DOI: 10.2210/pdb7w57/pdb
PDB entry 7w57 coloured by chain, front view.
PDB entry 7w57 coloured by chain, side view.
PDB entry 7w57 coloured by chain, top view.
Source organisms:
Primary publication:
Structural insights into the peptide selectivity and activation of human neuromedin U receptors.
You C, Zhang Y, Xu P, Huang S, Yin W, Eric Xu H, Jiang Y
Nat Commun 13 2045 (2022)
PMID: 35440625
Related structures: EMD-32316

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-209573 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 41.72 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63092 (Residues: 1-3, 27-38, 40-67, 205-251, 266-346, 348-357, 359-386, 389-394; Coverage: 55%)
  • Canonical: P63096 (Residues: 4-19, 32-32, 61-181, 229-242, 309-309, 319-319, 347-348; Coverage: 41%)
Gene names: GNAI1, GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 345 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Neuromedin-S Chain: C
Molecule details ›
Chain: C
Length: 34 amino acids
Theoretical weight: 3.93 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q5H8A3 (Residues: 109-141; Coverage: 26%)
Gene name: NMS
scFv16 Chain: E
Molecule details ›
Chain: E
Length: 248 amino acids
Theoretical weight: 26.5 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 70 amino acids
Theoretical weight: 7.73 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P59768 (Residues: 2-71; Coverage: 99%)
Gene name: GNG2
Sequence domains: GGL domain
Neuromedin-U receptor 2; Soluble cytochrome b562 Chain: R
Molecule details ›
Chain: R
Length: 571 amino acids
Theoretical weight: 65.42 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P0ABE7 (Residues: 23-127; Coverage: 99%)
  • Canonical: Q9GZQ4 (Residues: 1-415; Coverage: 100%)
Gene names: NMU2R, NMUR2, TGR1, cybC
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-32316
Expression system: Trichoplusia ni

Quick links

Citations

3 citation in other articles

Ligand recognition mechanism of the human relaxin family peptide receptor 4 (RXFP4).
Chen et al. (2023)
2 more