› 7rb8

Electron Microscopy
3.63Å resolution

cryo-EM structure of the ADP state wild type myosin-15-F-actin complex

Released:
DOI: 10.2210/pdb7rb8/pdb
PDB entry 7rb8 coloured by chain, front view.
PDB entry 7rb8 coloured by chain, side view.
PDB entry 7rb8 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for tunable control of actin dynamics by myosin-15 in mechanosensory stereocilia.
Gong R, Jiang F, Moreland ZG, Reynolds MJ, de Los Reyes SE, Gurel P, Shams A, Heidings JB, Bowl MR, Bird JE, Alushin GM
Sci Adv 8 eabl4733 (2022)
PMID: 35857845
Related structures: EMD-24399

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159346 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 373 amino acids
Theoretical weight: 41.63 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P68139 (Residues: 5-377; Coverage: 99%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Unconventional myosin-XV Chain: D
Molecule details ›
Chain: D
Length: 685 amino acids
Theoretical weight: 78.18 KDa
Source organism: Mus musculus
Expression system: Spodoptera aff. frugiperda 2 RZ-2014
UniProt:
  • Canonical: Q9QZZ4 (Residues: 1205-1889; Coverage: 20%)
Gene names: Myo15, Myo15a
Sequence domains: Myosin head (motor domain)

Ligands and Environments

2 bound ligands:
Ligand ADP 4 x ADP
Ligand MG 4 x MG
1 modified residue:
Ligand HIC 3 x HIC

Experiments and Validation Details

Entry percentile scores
Resolution: 3.63Å
Relevant EMDB volumes: EMD-24399
Expression system: Spodoptera aff. frugiperda 2 RZ-2014

Quick links

Citations

3 review citations

Myosin motors in sensory hair bundle assembly.
Moreland et al. (2022)
2 more

1 mention without citation

High-resolution structures of the actomyosin-V complex in three nucleotide states provide insights into the force generation mechanism.
Pospich et al. (2021)