7b2p

Electron Microscopy
3.43Å resolution

Cryo-EM structure of complement C4b in complex with nanobody B5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-209599 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Complement C4 beta chain Chain: A
Molecule details ›
Chain: A
Length: 656 amino acids
Theoretical weight: 71.76 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 20-675; Coverage: 38%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Complement C4-A alpha chain Chain: B
Molecule details ›
Chain: B
Length: 767 amino acids
Theoretical weight: 84.27 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 680-1446; Coverage: 45%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Complement C4 gamma chain Chain: C
Molecule details ›
Chain: C
Length: 291 amino acids
Theoretical weight: 33.12 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: P0C0L4 (Residues: 1454-1744; Coverage: 17%)
Gene names: C4A, CO4, CPAMD2
Sequence domains:
Nanobody B5 Chain: D
Molecule details ›
Chain: D
Length: 140 amino acids
Theoretical weight: 15.05 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.43Å
Relevant EMDB volumes: EMD-11989
Expression system: Escherichia coli BL21(DE3)