› 6ygm

X-ray diffraction
1.23Å resolution

TGT W95F mutant labelled mit 5F-Trp crystallised at pH 5.5

Released:
DOI: 10.2210/pdb6ygm/pdb
PDB entry 6ygm coloured by chain, front view.
PDB entry 6ygm coloured by chain, side view.
PDB entry 6ygm coloured by chain, top view.
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Entry authors: Nguyen A, Heine A, Klebe G

Function and Biology Details

Reaction catalysed: 
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151274 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Queuine tRNA-ribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.08 KDa
Source organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Expression system: Escherichia coli
UniProt:
  • Canonical: P28720 (Residues: 1-386; Coverage: 100%)
Gene names: ZMO0363, tgt
Sequence domains: Queuine tRNA-ribosyltransferase

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand FTR 3 x FTR

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 84.536Å b: 64.97Å c: 71.251Å
α: 90° β: 93.996° γ: 90°
R-values:
R R work R free
0.137 0.136 0.16
Expression system: Escherichia coli

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