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Revisiting pH-gated conformational switch. Complex HK853-RR468 D53A pH 5.3

X-ray diffraction
2Å resolution
PDB entry 6rh2 coloured by chain, front view.
PDB entry 6rh2 coloured by chain, side view.
PDB entry 6rh2 coloured by chain, top view.
Source organism: Thermotoga maritima
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Response regulatory domain-containing protein and histidine kinase
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Multimeric state: hetero tetramer
Accessible surface area: 28906.83 Å2
Buried surface area: 12457.17 Å2
Dissociation area: 2,601.7 Å2
Dissociation energy (ΔGdiss): 59.3 kcal/mol
Dissociation entropy (TΔSdiss): 14.49 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-194547

Macromolecules

histidine kinase
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.38 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZV7 (Residues: 232-489; Coverage: 53%)
Gene name: TM_0853
Pfam:
InterPro:
histidine kinase in PDB entry 6rh2, assembly 1, front view.
histidine kinase in PDB entry 6rh2, assembly 1, side view.
histidine kinase in PDB entry 6rh2, assembly 1, top view.
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Response regulatory domain-containing protein
Chains: C, D
Length: 122 amino acids
Theoretical weight: 13.8 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYT9 (Residues: 1-122; Coverage: 100%)
Gene name: TM_0468
Pfam: Response regulator receiver domain
InterPro:
Response regulatory domain-containing protein in PDB entry 6rh2, assembly 1, front view.
Response regulatory domain-containing protein in PDB entry 6rh2, assembly 1, side view.
Response regulatory domain-containing protein in PDB entry 6rh2, assembly 1, top view.
Molecule details ›

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