› 6omq

X-ray diffraction
1.86Å resolution

Crystal structure of PtmU3 complexed with PTM substrate

Released:
DOI: 10.2210/pdb6omq/pdb
PDB entry 6omq coloured by chain, front view.
PDB entry 6omq coloured by chain, side view.
PDB entry 6omq coloured by chain, top view.
Source organism: Streptomyces platensis
Primary publication:
Characterization and Crystal Structure of a Nonheme Diiron Monooxygenase Involved in Platensimycin and Platencin Biosynthesis.
Dong LB, Liu YC, Cepeda AJ, Kalkreuter E, Deng MR, Rudolf JD, Chang C, Joachimiak A, Phillips GN, Shen B
J Am Chem Soc 141 12406-12412 (2019)
PMID: 31291107

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100786 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidohydrolase-related domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 356 amino acids
Theoretical weight: 39.41 KDa
Source organism: Streptomyces platensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0A0UVH9 (Residues: 1-356; Coverage: 100%)
Sequence domains: Amidohydrolase

Ligands and Environments

3 bound ligands:
Ligand ACT 9 x ACT
Ligand MN 7 x MN
Ligand MZD 1 x MZD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 112.564Å b: 122.864Å c: 139.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.181 0.235
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Enzymatic Late-Stage Modifications: Better Late Than Never.
Romero et al. (2021)
1 more

1 mention without citation

Characterization and Crystal Structure of a Nonheme Diiron Monooxygenase Involved in Platensimycin and Platencin Biosynthesis.
Dong et al. (2019)