› 6mgj

X-ray diffraction
2Å resolution

Crystal structure of the catalytic domain from GH74 enzyme PoGH74 from Paenibacillus odorifer, apoenzyme

Released:
DOI: 10.2210/pdb6mgj/pdb
PDB entry 6mgj coloured by chain, front view.
PDB entry 6mgj coloured by chain, side view.
PDB entry 6mgj coloured by chain, top view.
Source organism: Paenibacillus odorifer
Primary publication:
Structural enzymology reveals the molecular basis of substrate regiospecificity and processivity of an exemplar bacterial glycoside hydrolase family 74 endo-xyloglucanase.
Arnal G, Stogios PJ, Asohan J, Skarina T, Savchenko A, Brumer H
Biochem J 475 3963-3978 (2018)
PMID: 30463871

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103664 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CBM3 domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 747 amino acids
Theoretical weight: 81.03 KDa
Source organism: Paenibacillus odorifer
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1R0YRH0 (Residues: 35-779; Coverage: 75%)
Gene name: BSK60_29080
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase

Ligands and Environments

5 bound ligands:
Ligand MG 9 x MG
Ligand PE3 12 x PE3
Ligand GOL 36 x GOL
Ligand PO4 1 x PO4
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 144 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 239.094Å b: 226.674Å c: 187.224Å
α: 90° β: 114.07° γ: 90°
R-values:
R R work R free
0.149 0.149 0.176
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Cell Surface Xyloglucan Recognition and Hydrolysis by the Human Gut Commensal Bacteroides uniformis.
Grondin et al. (2022)
1 more