› 6i2g

X-ray diffraction
1.5Å resolution

ALFA-tag binding nanobody (NbALFA) bound to ALFA-tag peptide.

Released:
DOI: 10.2210/pdb6i2g/pdb
PDB entry 6i2g coloured by chain, front view.
PDB entry 6i2g coloured by chain, side view.
PDB entry 6i2g coloured by chain, top view.
Source organisms:
Primary publication:
The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications.
Götzke H, Kilisch M, Martínez-Carranza M, Sograte-Idrissi S, Rajavel A, Schlichthaerle T, Engels N, Jungmann R, Stenmark P, Opazo F, Frey S
OpenAccess logo Nat Commun 10 4403 (2019)
PMID: 31562305

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-211750 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ALFA nanobody Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.6 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli
N7P-SER-ARG-LEU-GLU-GLU-GLU-LEU-ARG-ARG-ARG-LEU-THR-GLU-LPD Chain: B
Molecule details ›
Chain: B
Length: 15 amino acids
Theoretical weight: 1.93 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand LPD 1 x LPD

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 101.245Å b: 32.379Å c: 64.735Å
α: 90° β: 147.22° γ: 90°
R-values:
R R work R free
0.167 0.165 0.2
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

A guide to: generation and design of nanobodies.
Muyldermans S. (2021)
16 more

7 mentions without citation

Single-domain near-infrared protein provides a scaffold for antigen-dependent fluorescent nanobodies.
Oliinyk et al. (2022)
6 more