Structure analysis

Engineered glycolyl-CoA reductase comprising 8 mutations with bound NADP+

X-ray diffraction
2.579Å resolution
Assembly composition:
homo tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: homo tetramer
Accessible surface area: 55856.61 Å2
Buried surface area: 20344.24 Å2
Dissociation area: 3,160.94 Å2
Dissociation energy (ΔGdiss): 35.54 kcal/mol
Dissociation entropy (TΔSdiss): 16.07 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-173136
Assembly 2
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Multimeric state: homo tetramer
Accessible surface area: 56088.89 Å2
Buried surface area: 20634.28 Å2
Dissociation area: 3,169.71 Å2
Dissociation energy (ΔGdiss): 34.72 kcal/mol
Dissociation entropy (TΔSdiss): 16.08 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-173136
Assembly 3
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Multimeric state: homo tetramer
Accessible surface area: 55843.02 Å2
Buried surface area: 20839.24 Å2
Dissociation area: 3,139.27 Å2
Dissociation energy (ΔGdiss): 38.12 kcal/mol
Dissociation entropy (TΔSdiss): 16.07 kcal/mol
Symmetry number: 4
PDBe Complex ID: PDB-CPX-173136

Macromolecules

Chains: A, B, C, D, E, F, G, H, I, J
Length: 522 amino acids
Theoretical weight: 55.28 KDa
Source organism: Rhodopseudomonas palustris BisB18
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q21A49 (Residues: 1-464; Coverage: 100%)
Gene name: RPC_1174
Pfam: Aldehyde dehydrogenase family
InterPro:
CATH:

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