› 6evw

Electron Microscopy
4.4Å resolution

Cryo-EM structure of GMPCPP-microtubule co-polymerised with doublecortin

Released:
DOI: 10.2210/pdb6evw/pdb
PDB entry 6evw coloured by chain, front view.
PDB entry 6evw coloured by chain, side view.
PDB entry 6evw coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
The role of tubulin-tubulin lattice contacts in the mechanism of microtubule dynamic instability.
Manka SW, Moores CA
OpenAccess logo Nat Struct Mol Biol 25 607-615 (2018)
PMID: 29967541
Related structures: EMD-3961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135924 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tubulin alpha-1B chain Chains: A, C, E, J, K, L
Molecule details ›
Chains: A, C, E, J, K, L
Length: 438 amino acids
Theoretical weight: 48.78 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-438; Coverage: 97%)
Gene name: TUBA1B
Sequence domains:
Tubulin beta chain Chains: B, D, F, G, H, I
Molecule details ›
Chains: B, D, F, G, H, I
Length: 429 amino acids
Theoretical weight: 48.11 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-429; Coverage: 96%)
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand GTP 6 x GTP
Ligand MG 12 x MG
Ligand G2P 6 x G2P
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.4Å
Relevant EMDB volumes: EMD-3961

Quick links

Citations

11 review citations

The tubulin code and its role in controlling microtubule properties and functions.
Janke et al. (2020)
10 more

4 mentions without citation

Microtubule structure by cryo-EM: snapshots of dynamic instability.
Manka et al. (2018)
3 more