› 6a0z

X-ray diffraction
2.33Å resolution

Crystal structure of broadly neutralizing antibody 13D4 bound to H5N1 influenza hemagglutinin, HA head region

Released:
DOI: 10.2210/pdb6a0z/pdb
PDB entry 6a0z coloured by chain, front view.
PDB entry 6a0z coloured by chain, side view.
PDB entry 6a0z coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for the Broad, Antibody-Mediated Neutralization of H5N1 Influenza Virus.
Lin Q, Li T, Chen Y, Lau SY, Wei M, Zhang Y, Zhang Z, Yao Q, Li J, Li Z, Wang D, Zheng Q, Yu H, Gu Y, Zhang J, Chen H, Li S, Xia N
J Virol 92 (2018)
PMID: 29925655

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207990 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fibritin C-terminal domain-containing protein; Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 551 amino acids
Theoretical weight: 62.91 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q6DQ34 (Residues: 17-520; Coverage: 89%)
  • Canonical: M1E1E4 (Residues: 1-28; Coverage: 48%)
Gene name: HA
Sequence domains:
Structure domains: Hemagglutinin (Ha1 Chain); Chain: A; domain 1
Antibody 13D4, Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 224 amino acids
Theoretical weight: 23.89 KDa
Source organism: Mus musculus
Antibody 13D4, Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.63 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 72.245Å b: 72.665Å c: 76.704Å
α: 90° β: 93.37° γ: 90°
R-values:
R R work R free
0.174 0.172 0.218
Expression system: Trichoplusia ni

Quick links

Citations

1 review citation

Inducing broad-based immunity against viruses with pandemic potential.
Sette et al. (2022)
4 other articles

3 mentions without citation

Analysis of the conserved protective epitopes of hemagglutinin on influenza A viruses.
Jiao et al. (2023)
2 more