› 6tyy

X-ray diffraction
1.36Å resolution

Hedgehog autoprocessing mutant D46H

Released:
DOI: 10.2210/pdb6tyy/pdb
PDB entry 6tyy coloured by chain, front view.
PDB entry 6tyy coloured by chain, side view.
PDB entry 6tyy coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
General Base Swap Preserves Activity and Expands Substrate Tolerance in Hedgehog Autoprocessing.
Zhao J, Ciulla DA, Xie J, Wagner AG, Castillo DA, Zwarycz AS, Lin Z, Beadle S, Giner JL, Li Z, Li H, Banavali N, Callahan BP, Wang C
J Am Chem Soc 141 18380-18384 (2019)
PMID: 31682419

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169784 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein hedgehog Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02936 (Residues: 258-403; Coverage: 31%)
Gene names: CG4637, hh
Sequence domains: Hint module

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSD 1 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P61
Unit cell:
a: 78.381Å b: 78.381Å c: 37.351Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.17 0.195
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Intein Inhibitors as Novel Antimicrobials: Protein Splicing in Human Pathogens, Screening Methods, and Off-Target Considerations.
Wall et al. (2021)
1 more

2 mentions without citation

Hedgehog Autoprocessing: From Structural Mechanisms to Drug Discovery.
Kandel et al. (2022)
1 more