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Structure of the Type IV phosphorothioation-dependent restriction endonuclease ScoMcrA

X-ray diffraction
3.15Å resolution
PDB entry 5zmm coloured by chain, front view.
PDB entry 5zmm coloured by chain, side view.
PDB entry 5zmm coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Assembly composition:
homo hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: HNH nuclease domain-containing protein
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Multimeric state: homo hexamer
Accessible surface area: 140347.94 Å2
Buried surface area: 17255.01 Å2
Dissociation area: 2,320.95 Å2
Dissociation energy (ΔGdiss): 22.72 kcal/mol
Dissociation entropy (TΔSdiss): 16.58 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-192190

Macromolecules

HNH nuclease domain-containing protein
Chains: A, B, C, D, E, F
Length: 561 amino acids
Theoretical weight: 62.18 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9L0M9 (Residues: 2-560; Coverage: 100%)
Gene names: SCD82.02c, SCO4631
Pfam: HNH endonuclease
InterPro:
HNH nuclease domain-containing protein in PDB entry 5zmm, assembly 1, front view.
HNH nuclease domain-containing protein in PDB entry 5zmm, assembly 1, side view.
HNH nuclease domain-containing protein in PDB entry 5zmm, assembly 1, top view.
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