› 5tih

X-ray diffraction
2.44Å resolution

Structural basis for inhibition of erythrocyte invasion by antibodies to Plasmodium falciparum protein CyRPA

Released:
DOI: 10.2210/pdb5tih/pdb
PDB entry 5tih coloured by chain, front view.
PDB entry 5tih coloured by chain, side view.
PDB entry 5tih coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for inhibition of erythrocyte invasion by antibodies to Plasmodium falciparum protein CyRPA.
Chen L, Xu Y, Wong W, Thompson JK, Healer J, Goddard-Borger ED, Lawrence MC, Cowman AF
OpenAccess logo Elife 6 (2017)
PMID: 28195530

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-231401 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cysteine-rich protective antigen Chain: A
Molecule details ›
Chain: A
Length: 335 amino acids
Theoretical weight: 39.49 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8IFM8 (Residues: 30-362; Coverage: 100%)
Gene names: CyRPA, PF3D7_0423800, pfd1130w
Sequence domains: Cysteine-Rich Protective Antigen 6 bladed domain
CyRPA antibody Fab Heavy Chain Chain: H
Molecule details ›
Chain: H
Length: 215 amino acids
Theoretical weight: 23.34 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
CyRPA antibody Fab Light Chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.53 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ACT 3 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P212121
Unit cell:
a: 79.95Å b: 87.38Å c: 145.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.189 0.212
Expression systems:
  • Spodoptera frugiperda
  • Mus musculus

Quick links

Citations

9 review citations

The Molecular Basis of Erythrocyte Invasion by Malaria Parasites.
Cowman et al. (2017)
8 more

5 mentions without citation

The RH5-CyRPA-Ripr Complex as a Malaria Vaccine Target.
Ragotte et al. (2020)
4 more