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GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression.

Peter D, Weber R, Sandmeir F, Wohlbold L, Helms S, Bawankar P, Valkov E, Igreja C, Izaurralde E
Genes Dev 31 1147-1161 (2017)
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Cited: 43 times
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Abstract

The eIF4E homologous protein (4EHP) is thought to repress translation by competing with eIF4E for binding to the 5' cap structure of specific mRNAs to which it is recruited through interactions with various proteins, including the GRB10-interacting GYF (glycine-tyrosine-phenylalanine domain) proteins 1 and 2 (GIGYF1/2). Despite its similarity to eIF4E, 4EHP does not interact with eIF4G and therefore fails to initiate translation. In contrast to eIF4G, GIGYF1/2 bind selectively to 4EHP but not eIF4E. Here, we present crystal structures of the 4EHP-binding regions of GIGYF1 and GIGYF2 in complex with 4EHP, which reveal the molecular basis for the selectivity of the GIGYF1/2 proteins for 4EHP. Complementation assays in a GIGYF1/2-null cell line using structure-based mutants indicate that 4EHP requires interactions with GIGYF1/2 to down-regulate target mRNA expression. Our studies provide structural insights into the assembly of 4EHP-GIGYF1/2 repressor complexes and reveal that rather than merely facilitating 4EHP recruitment to transcripts, GIGYF1/2 proteins are required for repressive activity.

Articles - 5nvn mentioned but not cited (1)

  1. GIGYF1/2 proteins use auxiliary sequences to selectively bind to 4EHP and repress target mRNA expression. Peter D, Weber R, Sandmeir F, Wohlbold L, Helms S, Bawankar P, Valkov E, Igreja C, Izaurralde E. Genes Dev 31 1147-1161 (2017)


Reviews citing this publication (6)

  1. Getting to the Cores of Autism. Iakoucheva LM, Muotri AR, Sebat J. Cell 178 1287-1298 (2019)
  2. The Organizing Principles of Eukaryotic Ribosome Recruitment. Pelletier J, Sonenberg N. Annu Rev Biochem 88 307-335 (2019)
  3. Translation initiation by cap-dependent ribosome recruitment: Recent insights and open questions. Shirokikh NE, Preiss T. Wiley Interdiscip Rev RNA 9 e1473 (2018)
  4. Mechanistic Insights into MicroRNA-Mediated Gene Silencing. Duchaine TF, Fabian MR. Cold Spring Harb Perspect Biol 11 a032771 (2019)
  5. Dysregulated Translation in Neurodevelopmental Disorders: An Overview of Autism-Risk Genes Involved in Translation. Chen YC, Chang YW, Huang YS. Dev Neurobiol 79 60-74 (2019)
  6. Biochemical and Structural Insights into the Eukaryotic Translation Initiation Factor eIF4E. Volpon L, Osborne MJ, Borden KLB. Curr Protein Pept Sci 20 525-535 (2019)

Articles citing this publication (36)

  1. GIGYF2 and 4EHP Inhibit Translation Initiation of Defective Messenger RNAs to Assist Ribosome-Associated Quality Control. Hickey KL, Dickson K, Cogan JZ, Replogle JM, Schoof M, D'Orazio KN, Sinha NK, Hussmann JA, Jost M, Frost A, Green R, Weissman JS, Kostova KK. Mol Cell 79 950-962.e6 (2020)
  2. Ribosome collisions trigger cis-acting feedback inhibition of translation initiation. Juszkiewicz S, Slodkowicz G, Lin Z, Freire-Pritchett P, Peak-Chew SY, Hegde RS. Elife 9 e60038 (2020)
  3. A threonyl-tRNA synthetase-mediated translation initiation machinery. Jeong SJ, Park S, Nguyen LT, Hwang J, Lee EY, Giong HK, Lee JS, Yoon I, Lee JH, Kim JH, Kim HK, Kim D, Yang WS, Kim SY, Lee CY, Yu K, Sonenberg N, Kim MH, Kim S. Nat Commun 10 1357 (2019)
  4. 4EHP-independent repression of endogenous mRNAs by the RNA-binding protein GIGYF2. Amaya Ramirez CC, Hubbe P, Mandel N, Béthune J. Nucleic Acids Res 46 5792-5808 (2018)
  5. 4EHP and GIGYF1/2 Mediate Translation-Coupled Messenger RNA Decay. Weber R, Chung MY, Keskeny C, Zinnall U, Landthaler M, Valkov E, Izaurralde E, Igreja C. Cell Rep 33 108262 (2020)
  6. Translational control of ERK signaling through miRNA/4EHP-directed silencing. Jafarnejad SM, Chapat C, Matta-Camacho E, Gelbart IA, Hesketh GG, Arguello M, Garzia A, Kim SH, Attig J, Shapiro M, Morita M, Khoutorsky A, Alain T, Gkogkas CG, Stern-Ginossar N, Tuschl T, Gingras AC, Duchaine TF, Sonenberg N. Elife 7 e35034 (2018)
  7. A conserved CAF40-binding motif in metazoan NOT4 mediates association with the CCR4-NOT complex. Keskeny C, Raisch T, Sgromo A, Igreja C, Bhandari D, Weichenrieder O, Izaurralde E. Genes Dev 33 236-252 (2019)
  8. 4E-T-bound mRNAs are stored in a silenced and deadenylated form. Räsch F, Weber R, Izaurralde E, Igreja C. Genes Dev 34 847-860 (2020)
  9. The TUTase URT1 connects decapping activators and prevents the accumulation of excessively deadenylated mRNAs to avoid siRNA biogenesis. Scheer H, de Almeida C, Ferrier E, Simonnot Q, Poirier L, Pflieger D, Sement FM, Koechler S, Piermaria C, Krawczyk P, Mroczek S, Chicher J, Kuhn L, Dziembowski A, Hammann P, Zuber H, Gagliardi D. Nat Commun 12 1298 (2021)
  10. microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development. Mayya VK, Flamand MN, Lambert AM, Jafarnejad SM, Wohlschlegel JA, Sonenberg N, Duchaine TF. Nucleic Acids Res 49 4803-4815 (2021)
  11. GIGYF1 loss of function is associated with clonal mosaicism and adverse metabolic health. Zhao Y, Stankovic S, Koprulu M, Wheeler E, Day FR, Lango Allen H, Kerrison ND, Pietzner M, Loh PR, Wareham NJ, Langenberg C, Ong KK, Perry JRB. Nat Commun 12 4178 (2021)
  12. Gene-level analysis of rare variants in 379,066 whole exome sequences identifies an association of GIGYF1 loss of function with type 2 diabetes. Deaton AM, Parker MM, Ward LD, Flynn-Carroll AO, BonDurant L, Hinkle G, Akbari P, Lotta LA, Regeneron Genetics Center, DiscovEHR Collaboration, Baras A, Nioi P. Sci Rep 11 21565 (2021)
  13. Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA repression. Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E. Nucleic Acids Res 47 7035-7048 (2019)
  14. SARS-CoV-2 impairs interferon production via NSP2-induced repression of mRNA translation. Xu Z, Choi JH, Dai DL, Luo J, Ladak RJ, Li Q, Wang Y, Zhang C, Wiebe S, Liu ACH, Ran X, Yang J, Naeli P, Garzia A, Zhou L, Mahmood N, Deng Q, Elaish M, Lin R, Mahal LK, Hobman TC, Pelletier J, Alain T, Vidal SM, Duchaine T, Mazhab-Jafari MT, Mao X, Jafarnejad SM, Sonenberg N. Proc Natl Acad Sci U S A 119 e2204539119 (2022)
  15. The suppressive cap-binding complex factor 4EIP is required for normal differentiation. Terrao M, Marucha KK, Mugo E, Droll D, Minia I, Egler F, Braun J, Clayton C. Nucleic Acids Res 46 8993-9010 (2018)
  16. Molecular basis for GIGYF-Me31B complex assembly in 4EHP-mediated translational repression. Peter D, Ruscica V, Bawankar P, Weber R, Helms S, Valkov E, Igreja C, Izaurralde E. Genes Dev 33 1355-1360 (2019)
  17. The SARS-CoV-2 protein NSP2 impairs the silencing capacity of the human 4EHP-GIGYF2 complex. Zou L, Moch C, Graille M, Chapat C. iScience 25 104646 (2022)
  18. LeishIF4E1 Deletion Affects the Promastigote Proteome, Morphology, and Infectivity. Tupperwar N, Shrivastava R, Shapira M. mSphere 4 e00625-19 (2019)
  19. A newly identified Leishmania IF4E-interacting protein, Leish4E-IP2, modulates the activity of cap-binding protein paralogs. Tupperwar N, Meleppattu S, Shrivastava R, Baron N, Gilad A, Wagner G, Léger-Abraham M, Shapira M. Nucleic Acids Res 48 4405-4417 (2020)
  20. DAP5 enables main ORF translation on mRNAs with structured and uORF-containing 5' leaders. Weber R, Kleemann L, Hirschberg I, Chung MY, Valkov E, Igreja C. Nat Commun 13 7510 (2022)
  21. Epstein-Barr Virus BGLF2 commandeers RISC to interfere with cellular miRNA function. Campbell AM, De La Cruz-Herrera CF, Marcon E, Greenblatt J, Frappier L. PLoS Pathog 18 e1010235 (2022)
  22. capCLIP: a new tool to probe translational control in human cells through capture and identification of the eIF4E-mRNA interactome. Jensen KB, Dredge BK, Toubia J, Jin X, Iadevaia V, Goodall GJ, Proud CG. Nucleic Acids Res 49 e105 (2021)
  23. The EIF4E1-4EIP cap-binding complex of Trypanosoma brucei interacts with the terminal uridylyl transferase TUT3. Falk F, Kamanyi Marucha K, Clayton C. PLoS One 16 e0258903 (2021)
  24. Cullin-independent recognition of HHARI substrates by a dynamic RBR catalytic domain. Reiter KH, Zelter A, Janowska MK, Riffle M, Shulman N, MacLean BX, Tamura K, Chambers MC, MacCoss MJ, Davis TN, Guttman M, Brzovic PS, Klevit RE. Structure 30 1269-1284.e6 (2022)
  25. The eIF4E homolog 4EHP (eIF4E2) regulates hippocampal long-term depression and impacts social behavior. Wiebe S, Meng XQ, Kim SH, Zhang X, Lacaille JC, Aguilar-Valles A, Sonenberg N. Mol Autism 11 92 (2020)
  26. Chemical shift assignment of the viral protein genome-linked (VPg) from potato virus Y. Coutinho de Oliveira L, Volpon L, Osborne MJ, Borden KLB. Biomol NMR Assign 13 9-13 (2019)
  27. Regulation of the Golgi Apparatus by p38 and JNK Kinases during Cellular Stress Responses. Nordgaard C, Tollenaere MAX, Val AMD, Bekker-Jensen DB, Blasius M, Olsen JV, Bekker-Jensen S. Int J Mol Sci 22 9595 (2021)
  28. Susceptibility factor StEXA1 interacts with StnCBP to facilitate potato virus Y accumulation through the stress granule-dependent RNA regulatory pathway in potato. Chen R, Tu Z, He C, Nie X, Li K, Fei S, Song B, Nie B, Xie C. Hortic Res 9 uhac159 (2022)
  29. Yeast Smy2 and its human homologs GIGYF1 and -2 regulate Cdc48/VCP function during transcription stress. Lehner MH, Walker J, Temcinaite K, Herlihy A, Taschner M, Berger AC, Corbett AH, Dirac Svejstrup AB, Svejstrup JQ. Cell Rep 41 111536 (2022)
  30. Construction of a Diagnostic m7G Regulator-Mediated Scoring Model for Identifying the Characteristics and Immune Landscapes of Osteoarthritis. Hao L, Shang X, Wu Y, Chen J, Chen S. Biomolecules 13 539 (2023)
  31. Modulation of IGF1R Signaling Pathway by GIGYF1 in High Glucose-Induced SHSY-5Y Cells. Zhou T, Ma Y, Tang J, Guo F, Dong M, Wei Q. DNA Cell Biol 37 1044-1054 (2018)
  32. Molecular basis for GIGYF-TNRC6 complex assembly. Sobti M, Mead BJ, Stewart AG, Igreja C, Christie M. RNA 29 724-734 (2023)
  33. Synonymous codon usage regulates translation initiation. Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, Stasevich TJ, Rissland OS. Cell Rep 42 113413 (2023)
  34. Genetic Ablation of GIGYF1, Associated With Autism, Causes Behavioral and Neurodevelopmental Defects in Zebrafish and Mice. Ding Z, Huang G, Wang T, Duan W, Li H, Wang Y, Jia H, Yang Z, Wang K, Chu X, Kurtz-Nelson EC, Ahlers K, Earl RK, Han Y, Feliciano P, Chung WK, Eichler EE, Jiang M, Xiong B. Biol Psychiatry 94 769-779 (2023)
  35. Intrinsically disordered regions of tristetraprolin and DCP2 directly interact to mediate decay of ARE-mRNA. Maciej VD, Mateva N, Schwarz J, Dittmers T, Mallick M, Urlaub H, Chakrabarti S. Nucleic Acids Res 50 10665-10679 (2022)
  36. Low Expression of GIGYF1 Inhibits Metastasis, Proliferation, and Promotes Apoptosis and Autophagy of Gastric Cancer Cells. Zhu L, Yao Z, Luo Q, Liu Y, Zhao W, Shao C, Shao S, Cui F. Int J Med Sci 20 1038-1045 (2023)


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