5lfc

X-ray diffraction
3.2Å resolution

Crystal structure of Leuconostoc citreum NRRL B-1299 N-terminally truncated dextransucrase DSR-M

Released:

Function and Biology Details

Reaction catalysed:
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dextransucrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 1293 amino acids
Theoretical weight: 144.27 KDa
Source organism: Leuconostoc citreum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0A2H4A2M1 (Residues: 1-1293; Coverage: 100%)
Gene name: dsrV
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 105.84Å b: 128.71Å c: 229.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.248
Expression system: Escherichia coli BL21