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The NatB Acetyltransferase Complex Bound To CoA and MES

X-ray diffraction
2.4Å resolution
PDB entry 5k04 coloured by chain, front view.
PDB entry 5k04 coloured by chain, side view.
PDB entry 5k04 coloured by chain, top view.
Source organism: Candida albicans WO-1
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: N-terminal acetyltransferase B complex subunit MDM20 and N-acetyltransferase domain-containing protein
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Multimeric state: hetero dimer
Accessible surface area: 38916.42 Å2
Buried surface area: 6841.79 Å2
Dissociation area: 2,513.03 Å2
Dissociation energy (ΔGdiss): 16.73 kcal/mol
Dissociation entropy (TΔSdiss): 13.43 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-111415

Macromolecules

N-terminal acetyltransferase B complex subunit MDM20
Chain: A
Length: 750 amino acids
Theoretical weight: 87.55 KDa
Source organism: Candida albicans WO-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C4YFL7 (Residues: 1-745; Coverage: 100%)
Gene name: CAWG_01335
Pfam: N-acetyltransferase B complex (NatB) non catalytic subunit
InterPro:
N-terminal acetyltransferase B complex subunit MDM20 in PDB entry 5k04, assembly 1, front view.
N-terminal acetyltransferase B complex subunit MDM20 in PDB entry 5k04, assembly 1, side view.
N-terminal acetyltransferase B complex subunit MDM20 in PDB entry 5k04, assembly 1, top view.
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N-acetyltransferase domain-containing protein
Chain: B
Length: 170 amino acids
Theoretical weight: 19.91 KDa
Source organism: Candida albicans WO-1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C4YDZ9 (Residues: 1-170; Coverage: 90%)
Gene name: CAWG_00748
Pfam: Acetyltransferase (GNAT) family
InterPro:
N-acetyltransferase domain-containing protein in PDB entry 5k04, assembly 1, front view.
N-acetyltransferase domain-containing protein in PDB entry 5k04, assembly 1, side view.
N-acetyltransferase domain-containing protein in PDB entry 5k04, assembly 1, top view.
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